Copper induces apoptotic cell death through reactive oxygen species-triggered oxidative stress in the intertidal copepod Tigriopus japonicus
- Authors
- Rhee, Jae-Sung; Yu, In Tag; Kim, Bo-Mi; Jeong, Chang-Bum; Lee, Kyun-Woo; Kim, Min-Jung; Lee, Su-Jae; Park, Gyung Soo; Lee, Jae-Seong
- Issue Date
- May-2013
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Tigriopus japonicus; Copper; Cytotoxicity; Apoptosis; Oxidative stress; TUNEL assay; BrdU assay; p38 MAPK
- Citation
- AQUATIC TOXICOLOGY, v.132, pp.182 - 189
- Indexed
- SCIE
SCOPUS
- Journal Title
- AQUATIC TOXICOLOGY
- Volume
- 132
- Start Page
- 182
- End Page
- 189
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/26741
- DOI
- 10.1016/j.aquatox.2013.02.013
- ISSN
- 0166-445X
- Abstract
- The copepod, Tigriopus japonicus is an important model for toxicity testing. However, no attempt has been made in analyzing the effect of toxicants at the level of the ROS-mediated signal transduction pathway. To understand copper-induced cytotoxicity at the molecular level, we employed several cellular and biochemical assays after exposure to copper, and found a significant induction of enzyme activities of antioxidant proteins with increased intracellular reactive oxygen species (ROS) as well as an increase of TUNEL-positive cells, but a decrease of BrdU-positive cells. In addition, several important genes such as p38 MAPK, antioxidant-related genes, Hsps, and apoptosis-related genes were significantly modulated by copper exposure. Taken together, we suggest that copper-induced cytotoxicity is mediated by the formation of intracellular ROS and oxidative stress in T. japonicus. Whole body biochemical assays such as TUNEL- and BrdU-assay will provide a better understanding of cellular responses such as apoptosis and cell death upon cytotoxic exposure of copper in T. japonicus. (C) 2013 Elsevier B.V. All rights reserved.
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