Augmentation of rat skin flap viability by relaxin-expressing adenovirus
- Authors
- Lee, WJ; Yun, CO; Yun, IS; Kim, YO; Choi, IK; Yun, TJ; Rah, DK
- Issue Date
- Nov-2011
- Publisher
- WILEY
- Citation
- WOUND REPAIR AND REGENERATION, v.19, no.6, pp.709 - 717
- Indexed
- SCIE
SCOPUS
- Journal Title
- WOUND REPAIR AND REGENERATION
- Volume
- 19
- Number
- 6
- Start Page
- 709
- End Page
- 717
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/27662
- DOI
- 10.1111/j.1524-475X.2011.00733.x
- ISSN
- 1067-1927
- Abstract
- Relaxin (RLX) has multiple vascular actions, including vasodilation and angiogenesis, which occur via induction of vascular endothelial growth factor (VEGF) expression. We generated a RLX-expressing (dE1-RGD/lacZ/RLX) adenovirus and investigated whether it enhances skin flap survival. Thirty Sprague-Dawley rats were divided into three groups: RLX-expressing adenovirus group, control virus group, and phosphate-buffered saline (PBS) group. Two days before surgery and immediately after flap elevation, the caudally based flap that was 3 9 cm in size was subdermally injected with the dE1-RGD/lacZ/RLX virus (10(7) PFU), dE1-RGD/lacZ virus (10(7) PFU), or PBS. The surviving area of the flap and the amount of blood flow were measured. On postoperative day 10, CD31-positive vessels and VEGF protein expression were examined. We observed a significant increase in the survival area of the flap in the RLX group. Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. CD31-positive vessels and VEGF protein expression were significantly greater in the RLX group. Thus, administration of RLX-expressing adenovirus into elevated skin flaps increased VEGF expression, the number of capillaries, and blood flow to the flap, thereby improving skin flap survival.
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