Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zoneopen access

Authors
Kim, MinyoungRhee, HyunjoonKang, Ji YoonKim, Tae SongKwak, Rhokyun
Issue Date
Feb-2017
Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
Keywords
Bioengineering; Issue 120; ion concentration polarization; preconcentration; ion exchange membrane; overlimiting current; electroosmotic flow; electro-osmotic instability
Citation
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v.2017, no.120
Indexed
SCIE
SCOPUS
Journal Title
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume
2017
Number
120
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/2769
DOI
10.3791/55313
ISSN
1940-087X
Abstract
The ion concentration polarization (ICP) phenomenon is one of the most prevailing methods to preconcentrate low-abundance biological samples. The ICP induces a noninvasive region for charged biomolecules ( i.e., the ion depletion zone), and targets can be preconcentrated on this region boundary. Despite the high preconcentration performances with ICP, it is difficult to find the operating conditions of nonpropagating ion depletion zones. To overcome this narrow operating window, we recently developed a new platform for spatiotemporally fixed preconcentration. Unlike preceding methods that only use ion depletion, this platform also uses the opposite polarity of the ICP ( i.e., ion enrichment) to stop the propagation of the ion depletion zone. By confronting the enrichment zone with the depletion zone, the two zones merge together and stop. In this paper, we describe a detailed experimental protocol to build this spatiotemporally defined ICP platform and characterize the preconcentration dynamics of the new platform by comparing them with those of the conventional device. Qualitative ion concentration profiles and current-time responses successfully capture the different dynamics between the merged ICP and the stand-alone ICP. In contrast to the conventional one that can fix the preconcentration location at only similar to 5 V, the new platform can produce a target-condensed plug at a specific location in the broad ranges of operating conditions: voltage ( 0.5-100 V), ionic strength ( 1-100 mM), and pH ( 3.7-10.3).
Files in This Item
Go to Link
Appears in
Collections
서울 공과대학 > 서울 기계공학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kwak, Rho kyun photo

Kwak, Rho kyun
COLLEGE OF ENGINEERING (SCHOOL OF MECHANICAL ENGINEERING)
Read more

Altmetrics

Total Views & Downloads

BROWSE