pH-sensitive colorimetric polydiacetylene vesicles for urease sensing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jannah, Fadilatul | - |
dc.contributor.author | Kim, Jong-Man | - |
dc.date.accessioned | 2021-08-03T02:55:59Z | - |
dc.date.available | 2021-08-03T02:55:59Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 0143-7208 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/32818 | - |
dc.description.abstract | Urease is a virulence-factor enzyme found in various pathogenic bacteria. Many urinary-tract and gastro-intestinal diseases, including cancer, are related to urease activity, indicating that urease is critical to the human body. Herein, we report a colorimetric urease-sensing method using polydiacetylene vesicles. Due to the ammonia formed during urea hydrolyzation, the increase in local pH causes a color transition of the polydiacetylene vesicles from blue to red. Urease detection using this method is rapid, simple, sensitive, and economical. A minimum of 70 pU/ml of urease can be detected after 15 min of incubation, with a linearity range of 20 pU/ml-0.2 mU/ml. Urease detection in simulated gastric fluid was satisfactory, with a recovery percentage of 93.7-109.1%. The method was also used to screen the urease inhibitor acetohydroxamic acid, and the half-maximal inhibition value (IC50) was found to be 50 mM. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | pH-sensitive colorimetric polydiacetylene vesicles for urease sensing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Man | - |
dc.identifier.doi | 10.1016/j.dyepig.2019.04.072 | - |
dc.identifier.scopusid | 2-s2.0-85065490321 | - |
dc.identifier.wosid | 000473380500003 | - |
dc.identifier.bibliographicCitation | DYES AND PIGMENTS, v.169, pp.15 - 21 | - |
dc.relation.isPartOf | DYES AND PIGMENTS | - |
dc.citation.title | DYES AND PIGMENTS | - |
dc.citation.volume | 169 | - |
dc.citation.startPage | 15 | - |
dc.citation.endPage | 21 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Textiles | - |
dc.subject.keywordPlus | IN-GEL DETECTION | - |
dc.subject.keywordPlus | SEED-GERMINATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | Urease sensor | - |
dc.subject.keywordAuthor | Polydiacetylene vesicles | - |
dc.subject.keywordAuthor | pH-responsive sensor | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0143720819305248?via%3Dihub | - |
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