pH-sensitive colorimetric polydiacetylene vesicles for urease sensing
- Authors
- Jannah, Fadilatul; Kim, Jong-Man
- Issue Date
- Oct-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Urease sensor; Polydiacetylene vesicles; pH-responsive sensor
- Citation
- DYES AND PIGMENTS, v.169, pp.15 - 21
- Indexed
- SCIE
SCOPUS
- Journal Title
- DYES AND PIGMENTS
- Volume
- 169
- Start Page
- 15
- End Page
- 21
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/32818
- DOI
- 10.1016/j.dyepig.2019.04.072
- ISSN
- 0143-7208
- Abstract
- Urease is a virulence-factor enzyme found in various pathogenic bacteria. Many urinary-tract and gastro-intestinal diseases, including cancer, are related to urease activity, indicating that urease is critical to the human body. Herein, we report a colorimetric urease-sensing method using polydiacetylene vesicles. Due to the ammonia formed during urea hydrolyzation, the increase in local pH causes a color transition of the polydiacetylene vesicles from blue to red. Urease detection using this method is rapid, simple, sensitive, and economical. A minimum of 70 pU/ml of urease can be detected after 15 min of incubation, with a linearity range of 20 pU/ml-0.2 mU/ml. Urease detection in simulated gastric fluid was satisfactory, with a recovery percentage of 93.7-109.1%. The method was also used to screen the urease inhibitor acetohydroxamic acid, and the half-maximal inhibition value (IC50) was found to be 50 mM.
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