One-step delivery of a functional multi-layered cell sheet using a thermally expandable hydrogel with controlled presentation of cell adhesive proteins
- Authors
- Lee, Yu Bin; Lee, Joong-yup; Byun, Hayeon; Ahmad, Taufiq; Akashi, Mitsuru; Matsusaki, Michiya; Shin, Heungsoo
- Issue Date
- Jan-2018
- Publisher
- IOP PUBLISHING LTD
- Keywords
- 3D multi-layered cell sheet formation; layer-by-layer coating; scaffold-free tissue harvest; thermally expandable hydrogel; surface modification
- Citation
- BIOFABRICATION, v.10, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOFABRICATION
- Volume
- 10
- Number
- 2
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4750
- DOI
- 10.1088/1758-5090/aa9d43
- ISSN
- 1758-5082
- Abstract
- In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic (R)-based hydrogel at two different concentrations (1 and 5 mu gml(-1)) for stable adhesion of the multi-layered cell sheet, followed by polydopamine coating. In both conditions, a multi-layered cell sheet was stably formed. Then, the cell sheet on the hydrogel modified with 1 mu gml(-1) FN rapidly detached (> 90% efficiency) in response to the expansion of the hydrogel when temperature changed from 37 degrees C to 4 degrees C, while the other group had a reduced detachment due to excessive cell-hydrogel interaction. The multi-layered cell sheet was evident in cell-extracellular matrix and cell-cell junction formation, and bFGF was continuously secreted over 7 days of in vitro culture. The multi-layered transplanted to the mouse subcutaneous tissue also exhibited evidence of vascular ingrowth, which collectively suggest that the delivery system maintaining cellular functions is applicable for regenerative medicine.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4750)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.