Development and characterization of heparin-immobilized polycaprolactone nanofibrous scaffolds for tissue engineering using gamma-irradiationopen access
- Authors
- Jeong, Jin-Oh; Jeong, Sung In; Park, Jong-Seok; Gwon, Hui-Jeong; Ahn, Sung-Jun; Shin, Heungsoo; Lee, Jae Young; Lim, Youn-Mook
- Issue Date
- Jan-2017
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- RSC ADVANCES, v.7, no.15, pp.8963 - 8972
- Indexed
- SCIE
SCOPUS
- Journal Title
- RSC ADVANCES
- Volume
- 7
- Number
- 15
- Start Page
- 8963
- End Page
- 8972
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4828
- DOI
- 10.1039/c6ra20082f
- ISSN
- 2046-2069
- Abstract
- Polycaprolactone (PCL) has been considered a useful material for orthopedic devices and osseous implants because of its biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces that reduce initial cell viability. In this study, we fabricated surface-modified PCL nanofibers for tissue engineering using radiation technology. We supplemented the hydrophilicity of the PCL nanofibers by introducing 2-aminoethyl methacrylate (AEMA) through gamma-irradiation and subsequently immobilized heparin onto the nanofibers using the EDC/NHS reaction. The SEM images show that there is almost no change in the morphology of nanofibers after radiation grafting of AEMA and heparin-immobilization onto PCL nanofibers. The surface properties of the scaffolds were characterized by ATR-FTIR, XPS, and fluorescamine staining in order to confirm the successful grafting of AEMA onto the PCL nanofibers. Immobilization of heparin was also confirmed by the amide I (1650 cm(-1)) and amide II group (1550 cm(-1)) from ATR-FTIR. The amounts of heparin were drastically increased on the AEMA-PCL nanofibers as revealed by TBO assay. The initial cell viability of hMSCs was significantly increased on the AEMA grafted nanofibers but grew slowly on heparin-immobilized nanofibers. The cumulative release of bone morphogenetic protein-2 (BMP-2) was slow and continuous onto the heparin-immobilized nanofibers (18.13 +/- 3.87 mu g mL(-1)) compared to PCL nanofibers (20.25 +/- 1.45 mu g mL(-1)). Therefore, heparin-immobilized nanofibers may be a good tool for tissue engineering applications using radiation technology.
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