Introduction of N-cadherin-binding motif to alginate hydrogels for controlled stem cell differentiation
- Authors
- Lee, Jae Won; An, Hyoseok; Lee, Kuen Yong
- Issue Date
- Jul-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Cell-cell interaction; Low-density lipoprotein receptor-related protein 5; N-cadherin; Stem cell; Alginate hydrogel
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.155, pp.229 - 237
- Indexed
- SCIE
SCOPUS
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 155
- Start Page
- 229
- End Page
- 237
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5396
- DOI
- 10.1016/j.colsurfb.2017.04.014
- ISSN
- 0927-7765
- Abstract
- Control of stem cell fate and phenotype using biomimetic synthetic extracellular matrices (ECMs) is an important tissue engineering approach. Many studies have focused on improving cell-matrix interactions. However, proper control of cell-cell interactions using synthetic ECMs could be critical for tissue engineering, especially with undifferentiated stem cells. In this study, alginate hydrogels were modified with a peptide derived from the low-density lipoprotein receptor-related protein 5 (LRP5), which is known to bind to N-cadherin, as a cell-cell interaction motif. In vitro changes in the morphology and differentiation of mouse bone marrow stromal cells (D1 stem cells) cultured in LRP5-alginate hydrogels were investigated. LRP5-alginate gels successfully induced stem cell aggregation and enhanced chondrogenic differentiation of Dl stem cells, compared to RGD-alginate gels, at low cell density. This approach to tailoring synthetic biomimetic ECM5 using cell-cell interaction motifs may be critical in tissue engineering approaches using stem cells.
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