Hepatocyte growth factor-expressing adenovirus upregulates matrix metalloproteinase-1 expression in keloid fibroblasts
- Authors
- Jeon, Yeo Reum; Ahn, Hyo Min; Choi, Il Kyu; Yun, Chae Ok; Rah, Dong Kyun; Lew, Dae Hyun; Lee, Won Jai
- Issue Date
- Mar-2016
- Publisher
- WILEY-BLACKWELL
- Citation
- INTERNATIONAL JOURNAL OF DERMATOLOGY, v.55, no.3, pp.356 - 361
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF DERMATOLOGY
- Volume
- 55
- Number
- 3
- Start Page
- 356
- End Page
- 361
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5635
- DOI
- 10.1111/ijd.12965
- ISSN
- 0011-9059
- Abstract
- BackgroundKeloids are marked by an overabundance of extracellular matrix. The antifibrotic effect of hepatocyte growth factor (HGF) is achieved by increasing the expression of matrix metalloproteinases (MMPs) that drive extracellular matrix catabolism. As such, we cultivated an RGD-modified HGF-expressing adenovirus (dE1-RGD/lacZ/HGF) for introduction into keloid fibroblasts (KFs), looking at the subsequent impact on MMP-1 expression. MethodsKFs infected with either test virus as experimental group (dE1-RGD/lacZ/HGF) or its counterpart (dE1-RGD/lacZ) as control group were examined for HGF protein expression using an enzyme-linked immunosorbent assay (ELISA). Collagen (types I and III) and MMP-1 mRNA levels were also determined by reverse transcriptase-polymerase chain reaction, and ELISA was used to monitor MMP-1 protein expression. ResultsIn KFs harboring the test virus, high levels of HGF were induced at a multiplicity of infection ratio of 50 (3260.6162.7pg/ml) after 72hours of incubation. Furthermore, reverse transcriptase-polymerase chain reaction and ELISA confirmed that MMP-1 mRNA and protein expression rose significantly in KFs after transduction by the test virus (P<0.05). However, mRNA levels of collagen were unaffected by the experimental group. ConclusionThese results suggest that an HGF-expressing adenovirus may be therapeutic for keloids by increasing MMP-1 expression.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5635)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.