Quantitative RT-PCR for detection of eNOS expression in cardiovascular cells

Abstract

Nitric oxide (NO) produciton has emerged as a prominent protector against cardiovascular disease. Fro therapeutic dleivery in ischemic tissue, NO stimulates angiogenesis through mechanisms such as VEGF receptor upregulation, stimulation of endothelial cell proliferation, migration and inhibition of angiostatin release. The aim of this study was to examine endothelial NO synthase (eNOS) plasmid delivery to cardiovascular cells mediated by the reducible poly(amido ethylenimine) (SS-PAEI) polymer poly(amido triethylenetetramine) (SS-PATETA). Recently, SS-PAEIs were show to facilitate high gene expression without exhibiting any significant toxicity. Here we present a sensitive method for detecting eNOS gene delivery efficiency in vitro.