Simultaneous monitoring of the bioconversion from lysine to glutaric acid by ethyl chloroformate derivatization and gas chromatography-mass spectrometry
- Authors
- Han, Yeong-Hoon; Choi, Tae-Rim; Park, Ye-Lim; Song, Hun-Suk; Choi, Yong-Keun; Kim, Hyun-Joong; Bhatia, Shashi Kant; Gurav, Ranjit; Park, Kyungmoon; Park, See-Hyoung; Kim, Wooseong; Yang, Yung-Hun
- Issue Date
- 15-May-2020
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Glutaric acid; Metabolites; Gas chromatography-mass spectrometry; Ethyl chloroformate; Mass chromatograms; Analytical method
- Citation
- ANALYTICAL BIOCHEMISTRY, v.597
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 597
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/11710
- DOI
- 10.1016/j.ab.2020.113688
- ISSN
- 0003-2697
- Abstract
- Glutaric acid is a precursor of a plasticizer that can be used for the production of polyester amides, ester plasticizer, corrosion inhibitor, and others. Glutaric acid can be produced either via bioconversion or chemical synthesis, and some metabolites and intermediates are produced during the reaction. To ensure reaction efficiency, the substrates, intermediates, and products, especially in the bioconversion system, should be closely monitored. Until now, high performance liquid chromatography (HPLC) has generally been used to analyze the glutaric acid-related metabolites, although it demands separate time-consuming derivatization and non-derivatization analyses. To substitute for this unreasonable analytical method, we applied herein a gas chromatography - mass spectrometry (GC-MS) method with ethyl chloroformate (ECF) derivatization to simultaneously monitor the major metabolites. We determined the suitability of GC-MS analysis using defined concentrations of six metabolites (L-lysine, cadaverine, 5-aminovaleric acid, 2-oxoglutaric acid, glutamate, and glutaric acid) and their mass chromatograms, regression equations, regression coefficient values (R-2), dynamic ranges (mM), and retention times (RT). This method successfully monitored the production process in complex fermentation broth.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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