Metalloprotein/Nanoparticle Hybrid Material to Enhance the Stability of Electrochemical Signals

Abstract

In this study, a hybrid material composed of metalloproteins and nanoparticles was fabricated by the enzyme precipitate coating method to enhance the intensity and stability of the signals in bioelectronic devices. Formation of the material and immobilization on a gold substrate were confirmed by scanning electron microscopy and atomic force microscopy, for which the results were compared against those of metalloprotein-capped nanoparticles not subjected to the method. The electrochemical properties and stability of the hybrid material and those of metalloproteins without nanoparticles were compared and investigated by cyclic voltammetry. The experimental results indicate that the hybrid material enhanced electrochemical signals and had high stability. Thus, the method used in this study could be broadly applied as an alternative to fabricate bioelectronic devices, so as to overcome the problems of low signal intensity and stability in such devices.