Analysis of transgenic silkworms producing insulin-like growth factor-I in Bombyx mori
- Authors
- Seong, Jiyeon; Kim, Min-Jung; Lee, Soo Won; Yang, Hee Jin; Kong, Hong Sik; Kim, Keun-Chong; Suh, Dong Sang
- Issue Date
- Oct-2013
- Publisher
- SPRINGER
- Keywords
- Silkworm; Bombyx mori; Insulin like growth factor-I; Transgenic
- Citation
- GENES & GENOMICS, v.35, no.5, pp.623 - 630
- Journal Title
- GENES & GENOMICS
- Volume
- 35
- Number
- 5
- Start Page
- 623
- End Page
- 630
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/17033
- DOI
- 10.1007/s13258-013-0112-6
- ISSN
- 1976-9571
- Abstract
- Silkworms contain a powerful and effective fibroin promoter, which controls the expression of fibroin, a silk protein. The fibroin promoter and well-known characteristics of silkworm, the application of transgenic technique to silkworm will provide an excellent opportunity to mass-produce biomolecules. In this study, the production of recombinant human insulin like growth factor-I (rhIGF-I) in the silkworm system was designed. The method makes use of the microinjection technique and P element vector to transfer foreign genes into the chromosomes. We constructed the expression vector using the fibroin gene promoter and P element vector containing IGF-I gene (pFpIGF-I). We then microinjected this vector into eggs, and through PCR screening, transgenic silkworms were selected. We isolated and purified rhIGF-I from silkworm cocoons, returning a concentration of rhIGF-I of about 1,300 ng/g from transgenic silkworm cocoons. In a comparison of transgenic silkworm rhIGF-I and colostral IGF-I on cell proliferation, colostral IGF-I was better able to increase the proliferation rate of the cell line relative to the transgenic silkworm rhIGF-I, and showed a similar cell proliferation pattern. The anti-cancer effects of transgenic silkworm rhIGF-I were higher than that of colostral IGF-I on HeLa and SNU-C1 cancer cells. These results confirmed the construction of new transgenic silkworm strains producing rhIGF-I.
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