Novel strategy for enhancing productivity in L-DOPA synthesis: The electroenzymatic approach using well-dispersed L-tyrosine
- Authors
- Min, Kyoungseon; Kathavarayan, Thenmozhi; Park, Kyungmoon; Yoo, Young Je
- Issue Date
- Jun-2013
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Electroenzymology; Well dispersed L-tyrosine; L-DOPA; Productivity; Kinetics
- Citation
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.90, pp.87 - 90
- Journal Title
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
- Volume
- 90
- Start Page
- 87
- End Page
- 90
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/17115
- DOI
- 10.1016/j.molcatb.2013.01.027
- ISSN
- 1381-1177
- Abstract
- Although L-DOPA (L-3,4-dihydroxyphenylalanine) is widely used as a drug for Parkinson's disease, there are critical drawbacks in the commercial synthetic method such as low conversion rate, poor productivity, and long operational time. In order to overcome these limitations, a novel electroenzymatic system using tyrosinase/carbon nanopowder/polypyrrole composite as a working cathode was reported with the outstanding conversion rate up to 95.9%. However, the productivity was still limited due to a low solubility of the substrate L-tyrosine in aqueous phase. Herein, we demonstrated a novel strategy for enhancing the productivity by employing well-dispersed L-tyrosine as the substrate. When using well-dispersed L-tyrosine, not only the concentration of the substrate was increased to 90.6 gL(-1) in aqueous phase but also the productivity was enhanced up to 15.3 gL(-1) h(-1). We also determined kinetic parameters in the electroenzymatic system and the kinetic results revealed that the outstanding conversion rate was based on the fast electrical reduction of the by-product to L-DOPA. Thus the electroenzymatic synthesis using well-dispersed L-tyrosine can be a potential candidate as a novel process for L-DOPA synthesis. (C) 2013 Elsevier B.V. All rights reserved.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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