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Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli

Authors
Yeom, Chang-HunJeong, Hee-Jin
Issue Date
Sep-2021
Publisher
MDPI
Keywords
matrix metalloproteinase; recombinant antibody; Escherichia coli; soluble expression
Citation
MEDICINA-LITHUANIA, v.57, no.9
Journal Title
MEDICINA-LITHUANIA
Volume
57
Number
9
URI
https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/17701
DOI
10.3390/medicina57090981
ISSN
1010-660X
Abstract
Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.
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