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Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
- Authors
- Jeong, Hee-Jin; Dong, Jinhua; Ueda, Hiroshi
- Issue Date
- 1-Jan-2019
- Publisher
- MDPI
- Keywords
- influenza A; hemagglutinin; H1N1; Quenchbody; immunosensor
- Citation
- SENSORS, v.19, no.1
- Journal Title
- SENSORS
- Volume
- 19
- Number
- 1
- ISSN
- 1424-8220
- Abstract
- We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.
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Related Researcher
- Jeong, Hee-Jin
- Science & Technology (Biological and Chemical Engineering)