Development of an Amphiphilic Matrix for Immobilization of Candida antartica Lipase B for Biodiesel Production
- Authors
- Lee, Ko Woon; Min, Kyoungseon; Park, Kyungmoon; Yoo, Young Je
- Issue Date
- Jul-2010
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- amphiphilic matrix; Candida antartica lipase B (CALB); immobilization; biodiesel production
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.15, no.4, pp.603 - 607
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 15
- Number
- 4
- Start Page
- 603
- End Page
- 607
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/20760
- DOI
- 10.1007/s12257-009-3085-1
- ISSN
- 1226-8372
- Abstract
- Biodiesel has been greatly interested as an alternative fuel and is produced by a transesterification reaction of oil with alcohol. Recently, microbial lipases have been used for biodiesel production. Among the microbial lipase, immobilized Candida antartica lipase B (CALB) is the most widely used. However, CALB is unstable and shows low catalytic efficiency in the reaction media because the reaction media contains a high concentration of methanol and the lipase is also inhibited by the by-product glycerol. In this study, to overcome these limitations, we developed an amphiphilic matrix to immobilize CALB. The immobilized lipase in an amphiphilic matrix with 80% ethyltrimethoxysilane (ETMS) in tetramethoxysilane (TMOS) and pretreated with oil showed the highest specific activity and biodiesel conversion ratio; about 90% biodiesel conversion in 24 h at an initial molar ratio of 1: 1 (oil : methanol) with stepwise methanol feeding in order to adjust the net molar ratio to be 1 : 3.
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