Analysis and comparison of cloning methods for the preparation of repetitive polypeptides
- Authors
- Chu, Hun Su; Choi, Cha Yong; Won, Jong In
- Issue Date
- Sep-2008
- Publisher
- KOREAN INST CHEM ENGINEERS
- Keywords
- Repetitive Polypeptide; ELP; Recursive Directional Ligation (RDL); Controlled Cloning Method (CCM)
- Citation
- KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.25, no.5, pp.1125 - 1130
- Journal Title
- KOREAN JOURNAL OF CHEMICAL ENGINEERING
- Volume
- 25
- Number
- 5
- Start Page
- 1125
- End Page
- 1130
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/22671
- DOI
- 10.1007/s11814-008-0184-1
- ISSN
- 0256-1115
- Abstract
- Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biological fields including tissue engineering scaffolds, drug delivery systems, biomaterials, and DNA separation systems. The physical/chemical properties of the repetitive polypeptides can be improved by changing the composition of the repeated amino acid sequence. In this study, we introduced genetic methods for the production of repetitive polypeptides. By using recursive directional ligation (RDL) and controlled cloning method (CCM), multimerized genes were cloned and identified. Also, we compared the characteristics of recursive directional ligation (RDL) with those of controlled cloning method (CCM).
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Collections - College of Engineering > Chemical Engineering Major > 1. Journal Articles
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