Continuous cell-free protein synthesis using glycolytic intermediates as energy sources
- Authors
- Kim, Ho-Cheol; Kim, Tae-Wan; Park, Chang-Gil; Oh, In-Seok; Park, Kyungmoon; Kim, Dong-Myung
- Issue Date
- May-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- glycolysis; ATP regeneration; S12 extract; cell-free protein synthesis; fructose-1,6-bisphosphate
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.5, pp.885 - 888
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 5
- Start Page
- 885
- End Page
- 888
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/22746
- ISSN
- 1017-7825
- Abstract
- In this work, we demonstrate that glycolytic intermediates can serve as efficient energy sources to regenerate ATP during continuous-exchange cell-free (CECF) protein synthesis reactions. Through the use of an optimal energy source, approximately 10 mg/ml of protein was generated from a CECF protein synthesis reaction at greatly reduced reagent costs. Compared with the conventional reactions utilizing phosphoenol pyruvate as an energy source, the described method yields 10-fold higher productivity per unit reagent cost, making the techniques of CECF protein synthesis a more realistic alternative for rapid protein production.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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