Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment
- Authors
- Park, Sol Lee; Cho, Jang Yeon; Kim, Su Hyun; Bhatia, Shashi Kant; Gurav, Ranjit; Park, See-Hyoung; Park, Kyungmoon; Yang, Yung-Hun
- Issue Date
- Dec-2021
- Publisher
- MDPI
- Keywords
- poly(3-hydroxybutyrate); bioplastics; biodegradation; screening; Microbulbifer genus
- Citation
- POLYMERS, v.13, no.23
- Journal Title
- POLYMERS
- Volume
- 13
- Number
- 23
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/24356
- DOI
- 10.3390/polym13234257
- ISSN
- 2073-4360
- Abstract
- Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, Microbulbifer sp. SOL66 (94.18% 16S rRNA with similarity to Microbulbifer hydrolyticus) demonstrated the highest degradation activity among five newly screened Microbulbifer genus strains. Microbulbifer sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 degrees C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that Microbulbifer sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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