Simultaneous biocatalyst production and Baeyer-Villiger oxidation for bioconversion of cyclohexanone by recombinant Escherichia coli expressing cyclohexanone monooxygenase
- Authors
- Lee, WH; Park, YC; Lee, DH; Parif, K; Seo, JH; Park, KM
- Issue Date
- 2005
- Publisher
- HUMANA PRESS INC
- Keywords
- simultaneous biocatalyst production and Baeyer-Villiger oxidation; cyclohexanone monooxygenase; Escherichia coli; fed-batch process; epsilon-caprolactone
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.121, pp.827 - 836
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 121
- Start Page
- 827
- End Page
- 836
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25265
- ISSN
- 0273-2289
- Abstract
- Cyclohexanone monooxygenase (CHMO) catalyzing Baeyer-Villiger oxidation converts cyclic ketones into optically pure lactones, which have been used as building blocks inorganic synthesis. A recombinant Escherichia coli BL21(DE3)/pMM4 expressing CHMO originated from Acinetobacter sp. NCIB 9871 was used to produce F-caprolactone through a simultaneous biocatalyst production and Baeyer-Villiger oxidation (SPO) process. A fed-batch process was designed to obtain high cell density for improving production of epsilon-caprolactone. The fed-batch SPO process gave the best results, 10.2 g/L of epsilon-caprolactone and 0.34 g/(L (.) h) of productivity corresponding to a 10.5- and 3.4-fold enhancement compared with those of the batch SPO, respectively.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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