Characterization of the proteolytic activity of bacteria isolated from a rotating biological contactor
- Authors
- Park, IJ; Yoon, JC; Park, SJ; Kim, EH; Cho, YJ; Shin, KS
- Issue Date
- Jun-2003
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Keywords
- Aeromonas; Bacillus; proteolytic bacteria; rotating biological contactor
- Citation
- JOURNAL OF MICROBIOLOGY, v.41, no.2, pp.73 - 77
- Journal Title
- JOURNAL OF MICROBIOLOGY
- Volume
- 41
- Number
- 2
- Start Page
- 73
- End Page
- 77
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25968
- ISSN
- 1225-8873
- Abstract
- Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismords, B. subtilis, Aeromonas hydrophila and B. amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70degreesC and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two proteases in all isolates.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > Civil Engineering Major > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/25968)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.