Development of a glutaric acid production system equipped with stepwise feeding of monosodium glutamate by whole-cell bioconversion
- Authors
- Cha, H.-G.; Kim, H.T.; Park, S.-H.; Min, K.; Kong, Y.; Joo, J.C.; Yang, Y.-H.; Ahn, J.-O.; Lee, J.-S.; Park, K.
- Issue Date
- 1-Sep-2022
- Publisher
- Elsevier Inc.
- Keywords
- Glutaric acid; Indirect α-ketoglutaric acid supplying system; Monosodium glutamate (MSG); Stepwise feeding system; α-ketoglutaric acid
- Citation
- Enzyme and Microbial Technology, v.159
- Journal Title
- Enzyme and Microbial Technology
- Volume
- 159
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/27831
- DOI
- 10.1016/j.enzmictec.2022.110053
- ISSN
- 0141-0229
1879-0909
- Abstract
- In the bioproduction of glutaric acid, an emerging bioplastic monomer, α-ketoglutaric acid (α-KG) is required as an amine acceptor for 4-aminobutyrate aminotransferase (GabT)-driven conversion of 5-aminovalerate (5-AVA) to glutarate semialdehyde. Herein, instead of using expensive α-KG, an indirect α-KG supply system was developed using a relatively cheap alternative, monosodium glutamate (MSG), for L-glutamate oxidase (Gox)-based whole-cell conversion. Using 200 mM 5-AVA and 30 mM MSG initially with Gox, 67.1 mM of glutaric acid was produced. By applying the stepwise feeding strategy of MSG, the glutaric acid production capability was increased to 159.1 mM glutaric acid with a conversion yield of 79.6%. In addition, a buffer-free one-pot reaction from L-lysine was also applied in a 5 L bioreactor to evaluate its industrial applicability, resulting in a conversion yield of 54.2%. The system developed herein might have great potential for the large-scale, economically feasible production of glutaric acid by whole-cell conversion. © 2022 Elsevier Inc.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/27831)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.