Trap column-based intact mass spectrometry for rapid and accurate evaluation of protein molecular weightopen access
- Authors
- Lim, Gyu-Min; Kim, Byung-Gee; Jeong, Hee-Jin
- Issue Date
- 24-May-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- RSC ADVANCES, v.12, no.25, pp.15643 - 15651
- Journal Title
- RSC ADVANCES
- Volume
- 12
- Number
- 25
- Start Page
- 15643
- End Page
- 15651
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/29479
- DOI
- 10.1039/d2ra00429a
- ISSN
- 2046-2069
- Abstract
- The determination of the molecular weight (MW) of a protein using high-resolution mass spectrometry (MS) is a crucial tool used to confirm whether the protein was correctly expressed and adequately purified. However, a non-volatile buffer is normally used for protein purification and storage. Therefore, a pre-treatment step using ultrafiltration (UF) is required to exchange the buffer with a volatile buffer prior to the introduction of the protein sample into the MS equipment. This pre-treatment step is time-consuming. In this study, a trap column-based pre-treatment method applied in a nano-LC system was developed for rapid and convenient analysis of the MW of proteins. First, the trap column system was compared with the conventional UF treatment system and non-treatment system using bovine serum albumin. Subsequently, the trap column system was applied to analyze the MW of commercially available and lab-synthesized recombinant proteins. The intensity of the base peak and signal-to-noise ratio of the trap column-based pre-treated protein were higher than those of the UF-treated protein. Moreover, the entire automated procedure of the trap column-based system was conducted within 20 min, which confirms its use in versatile and accurate protein identification.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/29479)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.