Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Joo-Kyung | - |
dc.contributor.author | Yun, Hanool | - |
dc.contributor.author | Yeom, Chang-Hun | - |
dc.contributor.author | Kim, Eun-Jung | - |
dc.contributor.author | Kim, Wooseong | - |
dc.contributor.author | Lee, Chang-Soo | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Jeong, Hee-Jin | - |
dc.date.accessioned | 2022-12-19T00:41:25Z | - |
dc.date.available | 2022-12-19T00:41:25Z | - |
dc.date.created | 2022-12-19 | - |
dc.date.issued | 2022-12-02 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/30632 | - |
dc.description.abstract | Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 10(3) to 10(5) CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | SENSITIVE DETECTION | - |
dc.subject | PCR DETECTION | - |
dc.subject | COMBINATION | - |
dc.subject | GENERATION | - |
dc.subject | CELLS | - |
dc.title | Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeong, Hee-Jin | - |
dc.identifier.doi | 10.1039/d2ra05694a | - |
dc.identifier.scopusid | 2-s2.0-85144073974 | - |
dc.identifier.wosid | 000893152900001 | - |
dc.identifier.bibliographicCitation | RSC ADVANCES, v.12, no.53, pp.34660 - 34669 | - |
dc.relation.isPartOf | RSC ADVANCES | - |
dc.citation.title | RSC ADVANCES | - |
dc.citation.volume | 12 | - |
dc.citation.number | 53 | - |
dc.citation.startPage | 34660 | - |
dc.citation.endPage | 34669 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | PCR DETECTION | - |
dc.subject.keywordPlus | COMBINATION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | CELLS | - |
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