Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodiesopen access
- Authors
- Kim, Joo-Kyung; Yun, Hanool; Yeom, Chang-Hun; Kim, Eun-Jung; Kim, Wooseong; Lee, Chang-Soo; Kim, Byung-Gee; Jeong, Hee-Jin
- Issue Date
- 2-Dec-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- RSC ADVANCES, v.12, no.53, pp.34660 - 34669
- Journal Title
- RSC ADVANCES
- Volume
- 12
- Number
- 53
- Start Page
- 34660
- End Page
- 34669
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/30632
- DOI
- 10.1039/d2ra05694a
- ISSN
- 2046-2069
- Abstract
- Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 10(3) to 10(5) CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.
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- Appears in
Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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