Generation of a novel monoclonal antibody against inflammatory biomarker S100A8 using hybridoma technology
- Authors
- Kim, Jong-Pyo; Yun, Hanool; Kim, Eun-Jung; Kim, Yun-Gon; Lee, Chang-Soo; Ko, Byoung Joon; Kim, Byung-Gee; Jeong, Hee-Jin
- Issue Date
- 1-Jun-2023
- Publisher
- Springer Science and Business Media B.V.
- Keywords
- Hybridoma technology; Inflammation; Monoclonal antibody; MRP8; S100A8
- Citation
- BIOTECHNOLOGY LETTERS, v.45, no.5-6, pp 589 - 600
- Pages
- 12
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 45
- Number
- 5-6
- Start Page
- 589
- End Page
- 600
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31069
- DOI
- 10.1007/s10529-023-03364-0
- ISSN
- 0141-5492
1573-6776
- Abstract
- Objectives: S100A8 is highly expressed in several inflammatory and oncological conditions. To address the current lack of a reliable and sensitive detection method for S100A8, we generated a monoclonal antibody with a high binding affinity to human S100A8 to enable early disease diagnosis. Results: A soluble recombinant S100A8 protein with a high yield and purity was produced using Escherichia coli. Next, mice were immunized with recombinant S100A8 to obtain anti-human S100A8 monoclonal antibodies using hybridoma technology. Lastly, the high binding activity of the antibody was confirmed and its sequence was identified. Conclusions: This method, including the production of antigens and antibodies, will be useful for the generation of hybridoma cell lines that produce anti-S100A8 monoclonal antibodies. Moreover, the sequence information of the antibody can be used to develop a recombinant antibody for use in various research and clinical applications. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31069)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.