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Generation of a Recombinant scFv against Deoxycholic Acid and Its Conversion to a Quenchbody for One-Step Immunoassay
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Ueda, Hiroshi | - |
| dc.contributor.author | Jeong, Hee-Jin | - |
| dc.date.accessioned | 2023-11-10T07:43:49Z | - |
| dc.date.available | 2023-11-10T07:43:49Z | - |
| dc.date.issued | 2023-10 | - |
| dc.identifier.issn | 2409-9279 | - |
| dc.identifier.issn | 2409-9279 | - |
| dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/31842 | - |
| dc.description.abstract | Development of a rapid detection method for deoxycholic acid (DCA) is crucial for its diagnosis in the early stages of inflammation and cancer. In this study, we expressed a soluble recombinant anti-DCA single-chain variable fragment (scFv) in Escherichia coli. To convert scFv into a Quenchbody (Q-body), we labeled scFv using commercially available maleimide-linked fluorophores. The TAMRA-C5-maleimide-conjugated Q-body showed the highest response within a few minutes of DCA addition, indicating its applicability as a wash-free immunoassay probe for onsite DCA detection. © 2023 by the authors. | - |
| dc.language | 영어 | - |
| dc.language.iso | ENG | - |
| dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
| dc.title | Generation of a Recombinant scFv against Deoxycholic Acid and Its Conversion to a Quenchbody for One-Step Immunoassay | - |
| dc.type | Article | - |
| dc.publisher.location | 스위스 | - |
| dc.identifier.doi | 10.3390/mps6050090 | - |
| dc.identifier.scopusid | 2-s2.0-85175310059 | - |
| dc.identifier.wosid | 001099314400001 | - |
| dc.identifier.bibliographicCitation | Methods and Protocols, v.6, no.5 | - |
| dc.citation.title | Methods and Protocols | - |
| dc.citation.volume | 6 | - |
| dc.citation.number | 5 | - |
| dc.type.docType | Article | - |
| dc.description.isOpenAccess | Y | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.description.journalRegisteredClass | esci | - |
| dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
| dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
| dc.subject.keywordPlus | PHOTOINDUCED ELECTRON-TRANSFER | - |
| dc.subject.keywordPlus | CHAIN FV FRAGMENT | - |
| dc.subject.keywordPlus | MINI Q-BODY | - |
| dc.subject.keywordPlus | ANTIBODY | - |
| dc.subject.keywordPlus | RESIDUES | - |
| dc.subject.keywordPlus | BIOSENSORS | - |
| dc.subject.keywordPlus | MECHANISM | - |
| dc.subject.keywordPlus | DYES | - |
| dc.subject.keywordAuthor | deoxycholic acid | - |
| dc.subject.keywordAuthor | hapten | - |
| dc.subject.keywordAuthor | immunoassay | - |
| dc.subject.keywordAuthor | Quenchbody | - |
| dc.subject.keywordAuthor | recombinant antibody | - |
| dc.subject.keywordAuthor | single-chain variable fragment | - |
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Related Researcher
- Jeong, Hee-Jin
- Science & Technology (Biological and Chemical Engineering)