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Fluorogenic enzyme-linked immunosorbent assay with a dual color variation
- Authors
- Yun, Hanool; Jeong, Hee-Jin
- Issue Date
- 5-Apr-2024
- Publisher
- Elsevier B.V.
- Keywords
- Fluorescent biosensor; Fluorogenic enzyme-linked immunosorbent assay; Immunoassay; Programmed cell death-ligand 1; Tumor necrosis factor alpha
- Citation
- Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, v.310
- Journal Title
- Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
- Volume
- 310
- ISSN
- 1386-1425
1873-3557
- Abstract
- The development of accurate and high-throughput biomarker detection tools is crucial for the diagnosis, monitoring, and treatment of various diseases. In this study, a sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA) using Amplex Red or QuantaBlu fluorescent substrate was developed for the detection of tumor necrosis factor alpha and programmed cell death-ligand 1. The limit of detection of FELISA was in the nanogram order and multiple samples were conveniently assayed within 20 h using FELISA, demonstrating its applicability as a powerful immunoassay tool. FELISA can be widely used for rapid and accurate TNFα and PDL1 detection and applied to various fluorogenic immunoassays against other antigens of interest. © 2024 Elsevier B.V.
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Related Researcher
- Jeong, Hee-Jin
- Science & Technology (Biological and Chemical Engineering)