Evaluation of PDL1 positive cancer cell-specific binding activity of recombinant anti-PDL1 scFv
- Authors
- Kim, Sun-Hee; Park, Hae-Min; Jeong, Hee-Jin
- Issue Date
- 20-Feb-2024
- Publisher
- WILEY
- Keywords
- cellular immunoassay; ELISA; PDL1; recombinant antibody; western blotting
- Citation
- BIOTECHNOLOGY PROGRESS
- Journal Title
- BIOTECHNOLOGY PROGRESS
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/33032
- DOI
- 10.1002/btpr.3439
- ISSN
- 8756-7938
1520-6033
- Abstract
- Programmed cell death-ligand 1 (PDL1) is a transmembrane protein that is characterized as an immune regulatory molecule. We recently developed a recombinant single-chain fragment of variable domain (scFv) against PDL1, which showed high binding efficiency to purified recombinant PDL1 protein. However, at that time, proof-of-concept data for the effect of scFv using PDL1-expressing cells was lacking. In this study, we conducted two kinds of cell-based immunoassays, western blotting and enzyme-linked immunosorbent assay, using anti-PDL1 scFv. The results indicate that scFv can selectively and sensitively detect PDL1 from PDL1 positive human cancer cell lines. Our findings suggest that scFv could be used as a potential PDL1 inhibitor agent and probe for cell-based immunoassays to detect PDL1.
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