Establishment of efficient 5-hydroxyvaleric acid production system by regenerating alpha-ketoglutaric acid and its application in poly(5-hydroxyvaleric acid) production
- Authors
- Choi, Suhye; Kim, Byungchan; Kim, Suwon; Lee, Yeda; Shin, Yuni; Oh, Jinok; Bhatia, Shashi Kant; Seo, Seung-Oh; Park, See-Hyoung; Park, Kyungmoon; Yang, Yung-Hun
- Issue Date
- 20-May-2024
- Publisher
- Elsevier B.V.
- Keywords
- <sub>L-</sub>glutamate oxidase; 5-hydroxyvaleric acid; Catalase; Poly(5-hydroxyvaleric acid); Whole-cell biocatalysts; α-ketoglutaric acid
- Citation
- Journal of Biotechnology, v.387, pp 12 - 22
- Pages
- 11
- Journal Title
- Journal of Biotechnology
- Volume
- 387
- Start Page
- 12
- End Page
- 22
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/33077
- DOI
- 10.1016/j.jbiotec.2024.03.007
- ISSN
- 0168-1656
1873-4863
- Abstract
- 5-hydroxyvaleric acid (5-HV) is a versatile C5 intermediate of bio-based high-value chemical synthesis pathways. However, 5-HV production faces a few shortcomings involving the supply of cofactors, especially α-ketoglutaric acid (α-KG). Herein, we established a two-cell biotransformation system by introducing L-glutamate oxidase (GOX) to regenerate α-KG. Additionally, the catalase KatE was adapted to inhibit α-KG degradation by the H2O2 produced during GOX reaction. We searched for the best combination of genes and vectors and optimized the biotransformation conditions to maximize GOX effectiveness. Under the optimized conditions, 5-HV pathway with GOX showed 1.60-fold higher productivity than that of without GOX, showing 11.3 g/L titer. Further, the two-cell system with GOX and KatE was expanded to produce poly(5-hydroxyvaleric acid) (P(5HV)), and it reached at 412 mg/L of P(5HV) production and 20.5% PHA contents when using the biotransformation supernatant. Thus, the two-cell biotransformation system with GOX can potentially give the practical and economic alternative of 5-HV production using bio-based methods. We also propose direct utilization of 5-HV from bioconversion for P(5HV) production. © 2024 Elsevier B.V.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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