Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
DC Field | Value | Language |
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dc.contributor.author | Shin, Jihyun | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.contributor.author | Lee, Eunji | - |
dc.contributor.author | Hyun, Sung Min | - |
dc.contributor.author | Kim, Hyun Joong | - |
dc.contributor.author | Park, Si Jae | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Park, Kyungmoon | - |
dc.date.available | 2020-07-10T04:21:56Z | - |
dc.date.created | 2020-07-06 | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/3363 | - |
dc.description.abstract | Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | BACILLUS-CIRCULANS XYLANASE | - |
dc.subject | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject | GLUTAMATE-DECARBOXYLASE | - |
dc.subject | PLATFORM CHEMICALS | - |
dc.subject | THERMOSTABILIZATION | - |
dc.subject | ACID | - |
dc.subject | OPTIMIZATION | - |
dc.subject | BIOCATALYSTS | - |
dc.subject | STRESS | - |
dc.subject | DESIGN | - |
dc.title | Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyungmoon | - |
dc.identifier.doi | 10.1007/s12010-018-2696-4 | - |
dc.identifier.scopusid | 2-s2.0-85040972424 | - |
dc.identifier.wosid | 000439974900004 | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.185, no.4, pp.909 - 924 | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 185 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 909 | - |
dc.citation.endPage | 924 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | BACILLUS-CIRCULANS XYLANASE | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | GLUTAMATE-DECARBOXYLASE | - |
dc.subject.keywordPlus | PLATFORM CHEMICALS | - |
dc.subject.keywordPlus | THERMOSTABILIZATION | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | BIOCATALYSTS | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordAuthor | Cadaverine | - |
dc.subject.keywordAuthor | L-Lysine | - |
dc.subject.keywordAuthor | Lysine decarboxylase | - |
dc.subject.keywordAuthor | Whole-cell biotransformation | - |
dc.subject.keywordAuthor | Reusability | - |
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