Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
- Authors
- Shin, Jihyun; Joo, Jeong Chan; Lee, Eunji; Hyun, Sung Min; Kim, Hyun Joong; Park, Si Jae; Yang, Yung-Hun; Park, Kyungmoon
- Issue Date
- Aug-2018
- Publisher
- HUMANA PRESS INC
- Keywords
- Cadaverine; L-Lysine; Lysine decarboxylase; Whole-cell biotransformation; Reusability
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.185, no.4, pp.909 - 924
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 185
- Number
- 4
- Start Page
- 909
- End Page
- 924
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/3363
- DOI
- 10.1007/s12010-018-2696-4
- ISSN
- 0273-2289
- Abstract
- Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude l-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade l-lysine in comparison to EcCadA.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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