Fabrication of micrometer-scale porous gelatin scaffolds for 3D cell culture
- Authors
- Shim, Kyuyoung; Kim, So Hyun; Lee, Dongwook; Kim, Bumsang; Kim, Tae Hee; Jung, Youngmee; Choi, Nakwon; Sung, Jong Hwan
- Issue Date
- 25-Jun-2017
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Gelatin-methacrylate (Gel-MA); Micrometer-scale porous hydrogel scaffold; Photocrosslinking; Porogen; 3D cell culture
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.50, pp.183 - 189
- Journal Title
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Volume
- 50
- Start Page
- 183
- End Page
- 189
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/5603
- DOI
- 10.1016/j.jiec.2017.02.012
- ISSN
- 1226-086X
- Abstract
- In hydrogel scaffolds for 3D cell culture, porous structure plays a central role in maintaining cell survival by delivery of biochemical factors through the scaffolds. Thus, adequate pore structures should be a significant design factor for preparing cell culture scaffolds. Here, we present a non-cytotoxic approach to control porosity of a gelatin scaffold based on porogen leaching. Specifically, we fabricated photocrosslinkable hydrogel (gelatin methacrylate) containing porogens in a microfluidic channel, followed by dissolving out the porogens. Our approach allowed generation of micrometer-scale pores, increasing cell viability. This study could serve as a guide for tuning porosity of cell-seeded 3D scaffolds. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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Collections - College of Engineering > Chemical Engineering Major > 1. Journal Articles
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