Immobilization and stability studies of multi-thiol probes for electrical biosensors by label-free analysis
- Authors
- Kim, Won Jae; Shim, Min Suk; Chung, Minsub
- Issue Date
- 2017
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Biosensor; electrical detection; impedance spectroscopy; QCM-D; thiol immobilization
- Citation
- MOLECULAR CRYSTALS AND LIQUID CRYSTALS, v.654, no.1, pp.27 - 33
- Journal Title
- MOLECULAR CRYSTALS AND LIQUID CRYSTALS
- Volume
- 654
- Number
- 1
- Start Page
- 27
- End Page
- 33
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/6901
- DOI
- 10.1080/15421406.2017.1355192
- ISSN
- 1542-1406
- Abstract
- For electrical biosensors based on gold electrodes, poor long-term stability of the monothiol-to-gold bonds has been a major issue for a robust and reliable performance of the sensor. The use of multi-thiol modified bioprobes was proposed to achieve a stable attachment. In this study, the binding kinetics and stability of mono/dithiol modified oligonucleotides were investigated using quartz crystal microbalance with dissipation (QCM-D) and electrochemical impedance spectroscopy (EIS). These label-free surface tools compared the binding, stability and the effect of dithiothreitol in solution of monothiol, dithiol, or disulfide modification. The potential for development of label-free, sensitive DNA microarrays is demonstrated.
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Collections - College of Engineering > Chemical Engineering Major > 1. Journal Articles
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