Improved Transient Production of a Cellulase Enzyme in Detached Sunflower Leaves Using Plant Hormones
- Authors
- Jung, Sang-Kyu; McDonald, Karen A.
- Issue Date
- Nov-2016
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- agrobacterium; endoglucanase; jasmonic acid; phytohormone; sunflower; vacuum infiltration
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.21, no.6, pp.726 - 732
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 21
- Number
- 6
- Start Page
- 726
- End Page
- 732
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/7217
- DOI
- 10.1007/s12257-016-0410-3
- ISSN
- 1226-8372
- Abstract
- A synthetic endoglucanase E1 was transiently expressed in detached whole sunflower leaves using recombinant Agrobacterium tumefaciens. As a means to increase protein accumulation, the effect of plant hormones on E1 production was investigated using six different hormones: methyl jasmonic acid (JA), gibberellin A3 (GA), indole acetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (CK), and salicylic acid (SA). Among the six hormones, JA was best at boosting E1 concentrations, increasing yields as much as six-fold and the JA treatment was especially effective at an early phase of leaf incubation likely by enhancing T-DNA transfer at an early leaf incubation phase. Finally, co-infiltrating combinations of two different hormones revealed an antagonistic interaction between JA and SA in A. tumefaciens-mediated transient expression.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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