Medium engineering for enhanced production of undecylprodigiosin antibiotic in Streptomyces coelicolor using oil palm biomass hydrolysate as a carbon source
- Authors
- Bhatia, Shashi Kant; Lee, Bo-Rahm; Sathiyanarayanan, Ganesan; Song, Hun-Seok; Kim, Junyoung; Jeon, Jong-Min; Kim, Jung-Ho; Park, Sung-Hee; Yu, Ju-Hyun; Park, Kyungmoon; Yang, Yung-Hun
- Issue Date
- Oct-2016
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Antibiotic; Biosugar; Furfural; Streptomyces coelicolor; Undecylprodigiosin
- Citation
- BIORESOURCE TECHNOLOGY, v.217, pp.141 - 149
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 217
- Start Page
- 141
- End Page
- 149
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/7347
- DOI
- 10.1016/j.biortech.2016.02.055
- ISSN
- 0960-8524
- Abstract
- In this study, a biosugar obtained from empty fruit bunch (EFB) of oil palm by hot water treatment and subsequent enzymatic saccharification was used for undecylprodigiosin production, using Streptomyces coelicolor. Furfural is a major inhibitor present in EFB hydrolysate (EFBH), having a minimum inhibitory concentration (MIC) of 1.9 mM, and it reduces utilization of glucose (27%), xylose (59%), inhibits mycelium formation, and affects antibiotic production. Interestingly, furfural was found to be a good activator of undecylprodigiosin production in S. coelicolor, which enhanced undecylprodigiosin production by up to 52%. Optimization by mixture analysis resulted in a synthetic medium containing glucose: furfural: ACN: DMSO (1%, 2 mM, 0.2% and 0.3%, respectively). Finally, S. coelicolor was cultured in a fermenter in minimal medium with EFBH as a carbon source and addition of the components described above. This yielded 4.2 mu g/mg dcw undecylprodigiosin, which was 3.2-fold higher compared to that in un-optimized medium. (C) 2016 Elsevier Ltd. All rights reserved.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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