Post-Assembly Filling of Supported Lipid Bilayers by Soluble Lipid Derivatives
- Authors
- Chung, Minsub; Kim, Bumsang; Won, Jong-In
- Issue Date
- Aug-2016
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Supported lipid bilayers; Dye-labeled lipids; Defects; Self-quenching
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.37, no.8, pp.1354 - 1359
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 37
- Number
- 8
- Start Page
- 1354
- End Page
- 1359
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/7522
- DOI
- 10.1002/bkcs.10873
- ISSN
- 0253-2964
- Abstract
- Supported lipid bilayers (SLB) are widely used model biological membranes formed on glass or silica surfaces. Though subtle differences from the properties of free-standing membranes have been reported, its integrity and completeness is seldom questioned. In this report, we show that several different molecules, including some dye-labeled lipids, can insert from water into preformed and seemingly complete bilayers. The amount of insertion and the bilayer properties after filling were analyzed by a fluorescence bleaching/quenching experiment. The nature of this post-assembly saturation of SLB is discussed and supported by comparative experiments. This counter-intuitive but very reproducible result may help to further understand this model bilayer system.
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Collections - College of Engineering > Chemical Engineering Major > 1. Journal Articles
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