Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells
- Authors
- Park, See-Hyoung; Yoon, Young Ii; Moon, Hyoungwon; Lee, Ga-Hyun; Lee, Byung-Heon; Yoon, Tae-Jong; Lee, Hak Jong
- Issue Date
- Jul-2016
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- microbubble; liposome; ligand; IL4R; brain; tumor
- Citation
- ONCOLOGY REPORTS, v.36, no.1, pp.131 - 136
- Journal Title
- ONCOLOGY REPORTS
- Volume
- 36
- Number
- 1
- Start Page
- 131
- End Page
- 136
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/7648
- DOI
- 10.3892/or.2016.4836
- ISSN
- 1021-335X
- Abstract
- Gas (SF6)-filled microbubbles (MBs) were prepared by emulsion and solvent-evaporation method. The prepared MBs were further conjugated with doxorubicin (Dox)-loaded nano-sized liposome and peptide ligands to interleukin-4 receptor (IL4R) for targeting brain tumor cells. The final MB-liposome (Dox)-IL4R targeting peptide ligand [MB-Lipo (Dox)-IL4RTP] had a spherical structure with the mean size of 1,500 nm. The MB-Lipo (Dox)-IL4RTP exhibited cellular uptake in U87MG brain tumor cells (a brain tumor cell line expressing strongly IL4R) with frequency ultrasound energy suggesting that MB-Lipo (Dox)-IL4RTP provided effective targeting ability for brain tumor cells. In addition, WST-1 assay results showed that MB-Lipo (Dox)-IL4RTP inhibited the proliferation of U87MG cells IL4R-dependently. This was confirmed by western blotting of gamma H2AX, phospho (Ser15)-p53, p53 and p21 which are signal transduction proteins involved in DNA damage response and cell cycle arrest. Taken together, these results indicate that MB-Lipo (Dox)-IL4RTP represents a promising ultrasonic contrast agent for tumor targeting ultrasonic imaging.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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