Application of diethyl ethoxymethylenemalonate (DEEMM) derivatization for monitoring of lysine decarboxylase activity
- Authors
- Kim, Yong Hyun; Kim, Hyun Joong; Shin, Ji-Hyun; Bhatia, Shashi Kant; Seo, Hyung-Min; Kim, Yun-Gon; Lee, Yoo Kyung; Yang, Yung-Hun; Park, Kyungmoon
- Issue Date
- May-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Highly sensitive method; Diethyl ethoxymethylenemalonate; Diamine analysis; Cadevarine; Corynebacterium glutamicum
- Citation
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.115, pp.151 - 154
- Journal Title
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
- Volume
- 115
- Start Page
- 151
- End Page
- 154
- URI
- https://scholarworks.bwise.kr/hongik/handle/2020.sw.hongik/9937
- DOI
- 10.1016/j.molcatb.2015.01.018
- ISSN
- 1381-1177
- Abstract
- Aliphatic diamines have been used as chemical reagents for the monomers of polyamides (i.e., Nylon 46, Nylon 510, Nylon 66, Nylon 610 and so on), and can be made from lysine, arginine or ornithine by the metabolism of microbes through decarboxylases. However, the conventional derivatization methods for the HPLC-based quantitative analysis of aliphatic diamines exhibit poor sensitivity and efficiency for the monitoring of enzyme activity. In this study, a chemical derivatization method using diethyl ethoxymethylenemalonate (DEEMM) was applied to monitor lysine decarboxylase activity for the production of cadaverine by measuring the levels of intracellular and secreted lysine and cadaverine. The calibration graphs for the determination of 2,4-diaminobutyrate, 2,6-diaminopimelic acid, ornithine, arginine, lysine, 1,3-diaminopropane, putrescine dihydrochloride, cadaverine, hexamethylenediamine, and 1,7-diaminoheptane were measured by the highly sensitive method using DEEMM, and a linear relationship was observed for each dimaine compound, with limits of detection up to 0.001 mM. Application of this method will be useful for the sensitive monitoring of lysine decarboxylase reactions through the detection of substrates containing amine groups and products with diamines using HPLC and a UV detector. (C) 2015 Elsevier B.V. All rights reserved.
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Collections - College of Science and Technology > Department of Biological and Chemical Engineering > 1. Journal Articles
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