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Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain

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dc.contributor.authorHa, Chang Man-
dc.contributor.authorRyu, Young jae-
dc.contributor.authorKim, Yoonju-
dc.contributor.authorKim, Hyung-Jun-
dc.contributor.authorsung rae, Kim-
dc.contributor.authorSang-Joon Park-
dc.date.accessioned2023-11-17T01:00:04Z-
dc.date.available2023-11-17T01:00:04Z-
dc.date.issued2023-12-
dc.identifier.issn2409-9279-
dc.identifier.urihttp://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/1072-
dc.description.abstractWhole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing. Thus, many clearing methods have been developed to acquire whole-brain images or images of three-dimensional thick tissues. However, there are several limitations to imaging whole-brain volumes, including long image acquisition times, large volumes of data, and a long post-image process. Based on these limitations, many researchers are unsure about which light microscopy is most suitable for imaging thick tissues. Here, we compared fast-confocal microscopy with light-sheet fluorescence microscopy for whole-brain three-dimensional imaging, which can acquire images the fastest. To compare the two types of microscopies for large-volume imaging, we performed tissue clearing of a whole mouse brain, and changed the sample chamber and low- magnification objective lens and modified the sample holder of a light-sheet fluorescence microscope. We found out that light-sheet fluorescence microscopy using a 2.5× objective lens possesses several advantages, including saving time, large-volume image acquisitions, and high Z-resolution, over fast-confocal microscopy, which uses a 4× objective lens. Therefore, we suggest that light-sheet fluorescence microscopy is suitable for whole mouse brain imaging and for obtaining high-resolution three-dimensional images.-
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)-
dc.titleComparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain-
dc.typeArticle-
dc.publisher.location스위스-
dc.identifier.doi10.3390/mps6060108-
dc.identifier.scopusid2-s2.0-85180452590-
dc.identifier.wosid001131434400001-
dc.identifier.bibliographicCitationMethods and Protocols, v.6, no.6-
dc.citation.titleMethods and Protocols-
dc.citation.volume6-
dc.citation.number6-
dc.type.docTypeArticle-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClassesci-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.subject.keywordAuthorlight sheet fluorescence microscopy-
dc.subject.keywordAuthorfast confocal microscopy-
dc.subject.keywordAuthorthree-dimensional mouse brain imaging-
dc.subject.keywordAuthortissue clearing-
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연구본부 > 치매 연구그룹 > 1. Journal Articles
연구전략실 > 연구전략실 > 1. Journal Articles
연구전략실 > 첨단뇌연구장비센터 > 1. Journal Articles

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