Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brainopen accessComparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain
- Other Titles
- Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain
- Authors
- Ha, Chang Man; Ryu, Young jae; Kim, Yoonju; Kim, Hyung-Jun; sung rae, Kim; Sang-Joon Park
- Issue Date
- Nov-2023
- Publisher
- MDPI AG
- Keywords
- light sheet fluorescence microscopy; fast confocal microscopy; three-dimensional mouse brain imaging; tissue clearing
- Citation
- Methods and Protocols, v.6, no.6
- Journal Title
- Methods and Protocols
- Volume
- 6
- Number
- 6
- URI
- http://scholarworks.bwise.kr/kbri/handle/2023.sw.kbri/1072
- DOI
- 10.3390/mps6060108
- ISSN
- 2409-9279
2409-9279
- Abstract
- Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing. Thus, many clearing methods have been developed to acquire whole-brain images or images of three-dimensional thick tissues. However, there are several limitations to imaging whole-brain volumes, including long image acquisition times, large volumes of data, and a long post-image process. Based on these limitations, many researchers are unsure about which light microscopy is most suitable for imaging thick tissues. Here, we compared fast-confocal microscopy with light-sheet fluorescence microscopy for whole-brain three-dimensional imaging, which can acquire images the fastest. To compare the two types of microscopies for large-volume imaging, we performed tissue clearing of a whole mouse brain, and changed the sample chamber and low- magnification objective lens and modified the sample holder of a light-sheet fluorescence microscope. We found out that light-sheet fluorescence microscopy using a 2.5× objective lens possesses several advantages, including saving time, large-volume image acquisitions, and high Z-resolution, over fast-confocal microscopy, which uses a 4× objective lens. Therefore, we suggest that light-sheet fluorescence microscopy is suitable for whole mouse brain imaging and for obtaining high-resolution three-dimensional images.
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Collections - 연구본부 > 치매 연구그룹 > 1. Journal Articles
- 연구전략실 > 연구전략실 > 1. Journal Articles
- 연구전략실 > 첨단뇌연구장비센터 > 1. Journal Articles
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