Injectable Hydrogel Based on Protein-Polyester Microporous Network as an Implantable Niche for Active Cell Recruitment
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Phan, V. H. Giang | - |
dc.contributor.author | Murugesan, Mohanapriya | - |
dc.contributor.author | Manivasagan, Panchanathan | - |
dc.contributor.author | Thanh Loc Nguyen | - |
dc.contributor.author | Thuy-Hien Phan | - |
dc.contributor.author | Cuong Hung Luu | - |
dc.contributor.author | Duy-Khiet Ho | - |
dc.contributor.author | Li, Yi | - |
dc.contributor.author | Kim, Jaeyun | - |
dc.contributor.author | Lee, Doo Sung | - |
dc.contributor.author | Thambi, Thavasyappan | - |
dc.date.accessioned | 2024-02-27T16:31:41Z | - |
dc.date.available | 2024-02-27T16:31:41Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/28236 | - |
dc.description.abstract | Despite the potential of hydrogel-based localized cancer therapies, their efficacy can be limited by cancer recurrence. Therefore, it is of great significance to develop a hydrogel system that can provoke robust and durable immune response in the human body. This study has developed an injectable protein-polymer-based porous hydrogel network composed of lysozyme and poly(epsilon-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone-co-lactide (PCLA) (Lys-PCLA) bioconjugate for the active recruitment dendritic cells (DCs). The Lys-PCLA bioconjugates are prepared using thiol-ene reaction between thiolated lysozyme (Lys-SH) and acrylated PCLA (PCLA-Ac). The free-flowing Lys-PCLA bioconjugate sols at low temperature transformed to immovable gel at the physiological condition and exhibited stability upon dilution with buffers. According to the in vitro toxicity test, the Lys-PCLA bioconjugate and PCLA copolymer were non-toxic to RAW 263.7 cells at higher concentrations (1000 mu g/mL). In addition, subcutaneous administration of Lys-PCLA bioconjugate sols formed stable hydrogel depot instantly, which suggested the in situ gel forming ability of the bioconjugate. Moreover, the Lys-PCLA bioconjugate hydrogel depot formed at the interface between subcutaneous tissue and dermis layers allowed the active migration and recruitment of DCs. As suggested by these results, the in-situ forming injectable Lys-PCLA bioconjugate hydrogel depot may serve as an implantable immune niche for the recruitment and modification of DCs. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Injectable Hydrogel Based on Protein-Polyester Microporous Network as an Implantable Niche for Active Cell Recruitment | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/pharmaceutics14040709 | - |
dc.identifier.wosid | 000785217300001 | - |
dc.identifier.bibliographicCitation | PHARMACEUTICS, v.14, no.4 | - |
dc.citation.title | PHARMACEUTICS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 4 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | SUSTAINED-RELEASE | - |
dc.subject.keywordPlus | SITU | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordAuthor | thermo-responsive copolymers | - |
dc.subject.keywordAuthor | sol-gel phase transition | - |
dc.subject.keywordAuthor | injectable hydrogels | - |
dc.subject.keywordAuthor | protein-polymer conjugation | - |
dc.subject.keywordAuthor | immune cell recruitment | - |
dc.subject.keywordAuthor | dendritic cells | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
350-27, Gumi-daero, Gumi-si, Gyeongsangbuk-do, Republic of Korea (39253)054-478-7170
COPYRIGHT 2020 Kumoh University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.