Rapid and High-Density Antibody Immobilization Using Electropolymerization of Pyrrole for Highly Sensitive Immunoassay
DC Field | Value | Language |
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dc.contributor.author | Nam, Usun | - |
dc.contributor.author | Suh, Han Na | - |
dc.contributor.author | Sung, Sang-Keun | - |
dc.contributor.author | Seo, Chaewon | - |
dc.contributor.author | Lee, Jung Hyun | - |
dc.contributor.author | Lee, Jeong Yoon | - |
dc.contributor.author | Kim, Sanghee | - |
dc.contributor.author | Lee, Jukyung | - |
dc.date.accessioned | 2024-07-19T08:30:46Z | - |
dc.date.available | 2024-07-19T08:30:46Z | - |
dc.date.issued | 2024-06 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.issn | 1944-8252 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/28816 | - |
dc.description.abstract | Polypyrrole (Ppy) is a biologically compatible polymer that is used as a matrix, in which drugs and enzymes can be incorporated by doping. Here, we suggest an inventive application of Ppy as a biorecognition film encapsulated with an antibody (Ab) as an alternative strategy for the on-site multistep functionalization of thiol-based self-assembled monolayers. The fabrication steps of the recognition films were followed by dropping pyrrole and Ab mixed solutions onto the electrode and obtaining a thin film by direct current electropolymerization. The efficiency of Ab immobilization was studied by using fluorescence microscopy and electrochemical (EC) methods. Finally, the Ab density was increased and immobilized in 1 min, and the sensing performance as an EC immunosensor was demonstrated using alpha-fetoprotein with a limit of detection of 3.13 pg/mL and sensing range from 1 pg/mL to 100 ng/mL. This study demonstrates the potential for electrochemical functionalization of biomolecules with high affinity and rapidity. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Rapid and High-Density Antibody Immobilization Using Electropolymerization of Pyrrole for Highly Sensitive Immunoassay | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsami.4c00730 | - |
dc.identifier.scopusid | 2-s2.0-85196036312 | - |
dc.identifier.wosid | 001243972100001 | - |
dc.identifier.bibliographicCitation | ACS APPLIED MATERIALS & INTERFACES, v.16, no.24, pp 30611 - 30621 | - |
dc.citation.title | ACS APPLIED MATERIALS & INTERFACES | - |
dc.citation.volume | 16 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 30611 | - |
dc.citation.endPage | 30621 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | ALPHA-FETOPROTEIN | - |
dc.subject.keywordPlus | ELECTROCHEMICAL IMMUNOSENSORS | - |
dc.subject.keywordPlus | POLYPYRROLE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | 17-BETA-ESTRADIOL | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | ELECTRODES | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordAuthor | conducting polymers | - |
dc.subject.keywordAuthor | electropolymerization | - |
dc.subject.keywordAuthor | biorecognition | - |
dc.subject.keywordAuthor | polypyrrole (Ppy) | - |
dc.subject.keywordAuthor | alpha-fetoprotein(AFP) | - |
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