Rapid and High-Density Antibody Immobilization Using Electropolymerization of Pyrrole for Highly Sensitive Immunoassayopen access
- Authors
- Nam, Usun; Suh, Han Na; Sung, Sang-Keun; Seo, Chaewon; Lee, Jung Hyun; Lee, Jeong Yoon; Kim, Sanghee; Lee, Jukyung
- Issue Date
- Jun-2024
- Publisher
- AMER CHEMICAL SOC
- Keywords
- conducting polymers; electropolymerization; biorecognition; polypyrrole (Ppy); alpha-fetoprotein(AFP)
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.16, no.24, pp 30611 - 30621
- Pages
- 11
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 16
- Number
- 24
- Start Page
- 30611
- End Page
- 30621
- URI
- https://scholarworks.bwise.kr/kumoh/handle/2020.sw.kumoh/28816
- DOI
- 10.1021/acsami.4c00730
- ISSN
- 1944-8244
1944-8252
- Abstract
- Polypyrrole (Ppy) is a biologically compatible polymer that is used as a matrix, in which drugs and enzymes can be incorporated by doping. Here, we suggest an inventive application of Ppy as a biorecognition film encapsulated with an antibody (Ab) as an alternative strategy for the on-site multistep functionalization of thiol-based self-assembled monolayers. The fabrication steps of the recognition films were followed by dropping pyrrole and Ab mixed solutions onto the electrode and obtaining a thin film by direct current electropolymerization. The efficiency of Ab immobilization was studied by using fluorescence microscopy and electrochemical (EC) methods. Finally, the Ab density was increased and immobilized in 1 min, and the sensing performance as an EC immunosensor was demonstrated using alpha-fetoprotein with a limit of detection of 3.13 pg/mL and sensing range from 1 pg/mL to 100 ng/mL. This study demonstrates the potential for electrochemical functionalization of biomolecules with high affinity and rapidity.
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