Tat-CBR1 inhibits inflammatory responses through the suppressions of NF-kappa B and MAPK activation in macrophages and TPA-induced ear edema in mice
- Authors
- Kim, Young Nam; Kim, Dae Won; Jo, Hyo Sang; Shin, Min Jea; Ahn, Eun Hee; Ryu, Eun Ji; Yong, Ji In; Cha, Hyun Ju; Kim, Sang Jin; Yeo, Hyeon Ji; Youn, Jong Kyu; Hwang, Jae Hyeok; Jeong, Ji-Heon; Kim, Duk-Soo; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young
- Issue Date
- 15-Jul-2015
- Publisher
- Academic Press
- Keywords
- Apoptosis; Skin inflammation; Oxidative stress; Tat-CBR1; Protein therapy
- Citation
- Toxicology and Applied Pharmacology, v.286, no.2, pp 124 - 134
- Pages
- 11
- Journal Title
- Toxicology and Applied Pharmacology
- Volume
- 286
- Number
- 2
- Start Page
- 124
- End Page
- 134
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10461
- DOI
- 10.1016/j.taap.2015.03.020
- ISSN
- 0041-008X
1096-0333
- Abstract
- Human carbonyl reductase 1 (CBR1) plays a crucial role in cell survival and protects against oxidative stress response. However, its anti-inflammatory effects are not yet clearly understood. In this study, we examined whether CBR1 protects against inflammatory responses in macrophages and mice using a Tat-CBR1 protein which is able to penetrate into cells. The results revealed that purified Tat-CBR1 protein efficiently transduced into Raw 264.7 cells and inhibited lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2), nitric oxide (NO) and prostaglandin E-2 (PGE(2)) expression levels. In addition, Tat-CBR1 protein leads to decreased proinflammatory cytokine expression through suppression of nuclear transcription factor-kappaB (NF-kappa B) and mitogen activated protein kinase (MAPK) activation. Furthermore, Tat-CBR1 protein inhibited inflammatory responses in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation when applied topically. These findings indicate that Tat-CBR1 protein has anti-inflammatory properties in vitro and in vivo through inhibition of NF-kappa B and MAPK activation, suggesting that Tat-CBR1 protein may have potential as a therapeutic agent against inflammatory diseases. (C) 2015 Elsevier Inc. All rights reserved.
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Collections - College of Medicine > Department of Anatomy > 1. Journal Articles
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