Time-Dependent Changes of Cell Proliferation After Laser Photocoagulation in Mouse Chorioretinal Tissue
- Authors
- Lee, Si Hyung; Kim, Hoon Dong; Park, Yeo Jin; Ohn, Young-Hoon; Park, Tae Kwann
- Issue Date
- Apr-2015
- Publisher
- Association for Research in Vision and Ophthalmology
- Keywords
- cell proliferation; chorioretinal tissue; laser photocoagulation
- Citation
- Investigative Ophthalmology and Visual Science, v.56, no.4, pp 2696 - 2708
- Pages
- 13
- Journal Title
- Investigative Ophthalmology and Visual Science
- Volume
- 56
- Number
- 4
- Start Page
- 2696
- End Page
- 2708
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10748
- DOI
- 10.1167/iovs.14-16112
- ISSN
- 0146-0404
1552-5783
- Abstract
- PURPOSE. This study investigated the time course of cell proliferation after laser photocoagulation and analyzed the cell types of proliferating cells. METHODS. C57BL/6J mice received unilateral laser photocoagulation. Intraperitoneal bromodeoxyuridine (BrdU) injection was performed, and mice were divided into two groups according to the injection paradigm: group 1 with continuous injection and group 2 with periodic injection. Each group was again divided into four subgroups according to injection period: 0 to 3 days (n = 11), 0 to 7 days (n = 14), 0 to 14 days (n = 6), and 0 to 28 days (n = 6) after laser photocoagulation for group 1; and 0 to 3 days (n = 11), 4 to 7 days (n = 6), 8 to 14 days (n = 6), and 15 to 28 days (n = 6) after laser photocoagulation for group 2. The eyes were examined with immunohistochemistry using anti- BrdU antibody and other various antibodies for identification of proliferating cells. Manual cell counting and flow cytometry were performed for quantification. RESULTS. In group 1, the number of BrdU(+) cells showed marked increase during the first 3 days of laser lesioning, reaching its maximum after 7 days (P < 0.05). Group 2 also demonstrated peak proliferation during the first 3 days, but a significantly reduced number of BrdU(+) cells were detected during 4 to 7 days, 8 to 14 days, and 15 to 28 days of laser treatment (P < 0.05). BrdU(+) cells colocalized with CD11b, F4/80, iba1, RPE65, CD31, and glial fibrillary acidic protein (GFAP) labeling, and CD11b(+), F4/80(+), and iba1(+) cells constituted the main fraction of BrdU(+) cells. CONCLUSIONS. Laser photocoagulation induced cell proliferation mostly during the first 3 days, and many proliferating cells were identified as inflammatory cells, RPE cells, endothelial cells, and Muller cells.
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