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Platelet-rich plasma encapsulation in hyaluronic acid/gelatin-BCP hydrogel for growth factor delivery in BCP sponge scaffold for bone regeneration

Authors
Son, So-RaSarkar, Swapan KumarLinh, Nguyen-Thuy BaPadalhin, Andrew R.Kim, Bo RamJung, Hae IlLee, Byong-Taek
Issue Date
Feb-2015
Publisher
SAGE Publications
Keywords
Hyaluronic acid; gelatin; biphasic calcium phosphate; platelet-rich plasma; bone substitutes
Citation
Journal of Biomaterials Applications, v.29, no.7, pp 988 - 1002
Pages
15
Journal Title
Journal of Biomaterials Applications
Volume
29
Number
7
Start Page
988
End Page
1002
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10910
DOI
10.1177/0885328214551373
ISSN
0885-3282
1530-8022
Abstract
Microporous calcium phosphate based synthetic bone substitutes are used for bone defect healing. Different growth factor loading has been investigated for enhanced bone regeneration. The platelet is a cellular component of blood which naturally contains a pool of necessary growth factors that mediate initiation, continuation, and completion of cellular mechanism of healing. In this work, we have investigated the encapsulation and immobilization of platelet-rich plasma (PRP) with natural polymers like hyaluronic acid (HA) and gelatin (Gel) and loading them in a biphasic calcium phosphate (BCP) scaffold, for a synthetic-allologous hybrid scaffold. Effect of PRP addition in small doses was evaluated for osteogenic potential invitro and invivo. BCP (10%) mixed HA-Gel hydrogel with or without PRP, was loaded into a BCP sponge scaffold. We investigated the hydrogel-induced improvement in mechanical property and PRP-mediated enhancement in biocompatibility. Invitro studies for cytotoxicity, cell attachment, and proliferation were carried out using MC3T3-E1 pre-osteoblast cells. In invitro studies, the cell count, cell proliferation, and cell survival were higher in the scaffold with PRP loading than without PRP. However, in the invivo studies using a rat model, the PRP scaffold was not superior to the scaffold without PRP. This discrepancy was investigated in terms of the interaction of PRP in the invivo environment.
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