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Bacterial Identification and Detection of Equol in Korean Soybean PasteBacterial Identification and Detection of Equol in Korean Soybean Paste

Other Titles
Bacterial Identification and Detection of Equol in Korean Soybean Paste
Authors
Seung-Gyun WooSo-Yeon LeeGo-Woon ChoiYou-Jin HongSo-Min LeeKang Gyun Park엄용빈
Issue Date
2015
Publisher
대한임상검사과학회
Keywords
Equol; LC-MS/MS; MALDI-TOF mass spectrometry; 16S rRNA gene sequence
Citation
대한임상검사과학회지, v.47, no.4, pp 286 - 291
Pages
6
Journal Title
대한임상검사과학회지
Volume
47
Number
4
Start Page
286
End Page
291
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/11043
ISSN
1738-3544
2288-1662
Abstract
Equol has beneficial effects on human health. Fermented soy products contain equol, and many microbes participate in the equol production process. This study investigated fermented Korean soybean paste, doenjang. Thirty seven doenjang samples collected from different manufacturers were examined. Equol was detected in 3 samples (D2, D13, and D19) at the maximum content of 507 ng/100 g by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifteen microbial species were isolated and identified by 16S rRNA gene sequence analysis and by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Bacillus spp, Paenibacillus spp, Tetragenococcus spp, Stapylococcus spp, and Clostridium species were the predominant bacteria in equol containing doenjang samples.
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