Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes
- Authors
- Park, Mi-Young; Mun, Seong Taek
- Issue Date
- Oct-2014
- Publisher
- 한국영양학회
- Keywords
- 3T3-L1 adipocytes; carnosic acid; inflammation; toll-like receptor 4
- Citation
- Nutrition Research and Practice, v.8, no.5, pp 516 - 520
- Pages
- 5
- Journal Title
- Nutrition Research and Practice
- Volume
- 8
- Number
- 5
- Start Page
- 516
- End Page
- 520
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/11824
- DOI
- 10.4162/nrp.2014.8.5.516
- ISSN
- 1976-1457
2005-6168
- Abstract
- BACKGROUND/OBJECTIVES: Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes. MATERIALS/METHODS: 3T3-L1 adipocytes were treated with CA (0-20 mu M) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured. RESULTS: LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNE)-alpha, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-kappa B, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA. CONCLUSIONS: Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.
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Collections - College of Medicine > Department of Obstetrics and Gynecology > 1. Journal Articles
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